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环境科学学报 2009
Renaturation and degradation of thermo-treated waste plasmid DNA in an aquatic-environment
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Abstract:
Plasmid pET-28b was used as a test plasmid to investigate the potential environmental risk caused by thermo-treated recombinant DNA. Firstly,plasmid DNA transformation was performed to evaluate the re-naturation efficiency of the thermo-treated plasmid DNA,and then simulated aquatic environments were constructed to determine the persistence of un-decayed plasmid DNAs in the aquatic environment after thermo-treatment. The results showed that the transforming activity of the thermo-treated plasmid DNA could b...
