%0 Journal Article
%T Renaturation and degradation of thermo-treated waste plasmid DNA in an aquatic-environment<br>热处理重组质粒DNA的复性及其在水环境中的降解特性
%A LI Mengnan
%A ZHENG Guanghong
%A WANG Lei
%A FU Xiaohu
%A XIAO Wei
%A LE Yiquan
%A REN Daming
%A <br>李梦南
%A 郑广宏
%A 王磊
%A 付小花
%A 肖伟
%A 乐毅全
%A 任大明
%J 环境科学学报
%D 2009
%I 
%X Plasmid pET-28b was used as a test plasmid to investigate the potential environmental risk caused by thermo-treated recombinant DNA. Firstly,plasmid DNA transformation was performed to evaluate the re-naturation efficiency of the thermo-treated plasmid DNA,and then simulated aquatic environments were constructed to determine the persistence of un-decayed plasmid DNAs in the aquatic environment after thermo-treatment. The results showed that the transforming activity of the thermo-treated plasmid DNA could b...
%K thermo-treatment
%K plasmid DNA
%K stimulated aquatic environment
%K renaturation
%K transformation
%K degradation<br>热处理
%K 质粒DNA
%K 水环境
%K 复性
%K 转化
%K 降解
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=43E6659BA5E4CFE449375FFC3391E9FE&yid=DE12191FBD62783C&vid=771469D9D58C34FF&iid=9CF7A0430CBB2DFD&sid=ABD3F52B9C9810A2&eid=EFCEB529E333AB35&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=15