Effects of low-dose and long-term lead (Pb) exposure on testicular enzymes in male Rana nigromaculata
低剂量长期铅暴露对雄性黑斑蛙精巢组织关键酶活性的影响

To evaluate the reproductive toxicity of low-dose and long-term lead(Pb) exposure in male Rana nigromaculata,healthy adult male frogs were exposed to 0.1,0.2,0.4,0.8 and 1.6 mg · L-1 of lead solution for 30 d under experimental conditions.The activities of Ca2+-ATPase,Na+-K+-ATPase,Ca2+-Mg2+-ATPase,β-DG,LDH and ACP were tested.The results showed that the activities of Ca2+-ATPase and Na+-K+-ATPase were induced with the increasing lead level,while Ca2+-Mg2+-ATPase being inhibited.When the decrease of Ca2+-Mg2+-ATPase exceeded the increase of Ca2+-ATPase and Na+-K+-ATPase,the quantity of sperm decreased and the male reproductive toxicity of the frog was induced by lead.In addition,the activities of ACP were inhibited under 1.6 mg · L-1 Pb2+,implying decrease of the quantity of sperm because of damage of sertoli cell.Also,the activities of LDH were significantly inhibited under 0.4 mg · L-1 Pb2+,implying decrease of the quality of sperm because of the damage of spermatogenic cells.