Non-induced expression of the gene for triphenylmethane dye oxidase (tpmD) in E.coli
三苯基甲烷类染料氧化酶基因（tpmD）在大肠杆菌中的无诱导表达

According to the tpmD gene sequence of Aeromonas hydrophila DN322,a set of primers was designed,the forward primer containing a SD sequence before start codon ATG,and the reverse primer containing 18 bp coding for CCPGCC before the stop codon.This CCPGCC tag can covalently bind biarsenical MAPs,i.e.,fluorescein and other dyes with the xanthene backbone derivatized with two As(III) moieties.The PCR product was 919 bp,and named tpmD'.According to the Shewanella sp.ANA-3 NAD(P)H dehydrogenase gene promoter seq...