%0 Journal Article
%T Non-induced expression of the gene for triphenylmethane dye oxidase (tpmD) in E.coli<br>三苯基甲烷类染料氧化酶基因（tpmD）在大肠杆菌中的无诱导表达
%A WEI Jinbo
%A SUN Guoping
%A REN Suizhou
%A CEN Yinghua
%A WANG YanSouth China Sea Institute of Oceanography
%A Chinese Academy of Sciences
%A Guangzhou Guangdong Institute of Microbiology
%A Guangdong Provincial Key Laboratory of Microbial Culture Collection
%A Application
%A Guangzhou Graduate University of Chinese Academy of Sciences
%A Beijing The Science
%A Technology Library of Guangdong Province
%A Guangzhou
%A <br>卫晋波
%A 孙国萍
%A 任随周
%A 岑英华
%A 王燕
%J 环境科学学报
%D 2009
%I 
%X According to the tpmD gene sequence of Aeromonas hydrophila DN322,a set of primers was designed,the forward primer containing a SD sequence before start codon ATG,and the reverse primer containing 18 bp coding for CCPGCC before the stop codon.This CCPGCC tag can covalently bind biarsenical MAPs,i.e.,fluorescein and other dyes with the xanthene backbone derivatized with two As(III) moieties.The PCR product was 919 bp,and named tpmD'.According to the Shewanella sp.ANA-3 NAD(P)H dehydrogenase gene promoter seq...
%K SND Promoter
%K tpmD
%K triphenylmethane dyes
%K non-induction expression<br>嗜水气单胞菌脱色酶基因(tpmD)
%K 三苯基甲烷染料
%K 希瓦氏菌启动子
%K 无诱导表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=56D55DECFD2C61CB0F386B9BDFD3CF79&yid=DE12191FBD62783C&vid=771469D9D58C34FF&iid=38B194292C032A66&sid=F3549E0657848E2A&eid=A726E84831FE609B&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=0