Effect of 12-dimethylbenz(a)athrancene and benzo(a)pyrene on metallothionein expression and lipid peroxidation in mouse liver
二甲基苯蒽和苯并(a)芘对小鼠肝脏金属硫蛋白及氧化损伤的诱导作用

To study the induction of metallothionein (MT) and lipid peroxidation by two polycyclic aromatic hydrocarbons (PAHs), 7,12 dimethylbenza]athrancene (DMBA) and benzoa]pyrene (BaP) in mice liver. Male mice were treated with DMBA or BaP i.p. at the dose of 50?mg/kg body weight for 0, 24, 48, 72 and 144?h, respectively. Liver MT was measured by the cadmium/hemoglobin saturation assay. Lipid peroxidation product (malondialdehyde, MDA) was measured as thiobarbituric acid reactive substances. After DMBA or BaP treatment, mice liver weight, MT and MDA increased in amount. Liver boby weight ratio increased significantly in a time dependent manner. MT was significantly induced vs control after 24?h with PAHs treatment. Liver MDA level was elevated one fold after 24?h treatment. Liver MT has been induced by PAHs. The correlation between MT expression and MDA level suggest that MT may be related with oxidative stress caused by PAHs.