Analysis of nitrifying bacteria in short-cut nitrification-denitrification processes by using FISH, PCR-DGGE and Cloning
采用FISH、DGGE和Cloning对短程脱氮系统中硝化菌群的比较分析

Molecular biology techniques of Fish, PCR-DGGE and PCR-Cloning-Sequencing were used to qualitatively and quantitatively analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in SBR large pilot-scale plant, UASB-A/O lab-scale reactor, A/O pilot-scale plant and SBR lab-scale reactor with the performance of short-cut nitrification-denitrification treating actual wastewater. Fish results showed that in the above 4 processes, AOB became dominant with compared to NOB and accounted for 3%~12% of total biomass. No NOB was detected in both SBR pilot-scale and lab-scale reactors. A very small amount of Nitrospira and Nitrobacteria below 0.2% was found in A/O pilot-scale plant and UASB-A/O lab-scale reactor, respectively. PCR-DGGE results showed that the AOB in SBR pilot-scale, A/O pilot-scale and UASB-A/O lab-scale processes were phylogenetically related to Nitrosomonas-like species with a melting point in the range 30% to 50%. PCR-Cloning-Sequencing results showed that all clones from the sludge sample of the SBR large pilot-scale plant were affiliated with Nitrosomonas and 60% of the clones were affiliated with Nitrosomonas europaea.