%0 Journal Article %T Analysis of nitrifying bacteria in short-cut nitrification-denitrification processes by using FISH, PCR-DGGE and Cloning
采用FISH、DGGE和Cloning对短程脱氮系统中硝化菌群的比较分析 %A ZENG Wei %A YANG Qing %A ZHANG Shujun %A MA Yong %A LIU Xiuhong %A PENG Yongzhen %A LI Jun %A
曾薇 %A 杨庆 %A 张树军 %A 马勇 %A 刘秀红 %A 彭永臻 %A 李军 %J 环境科学学报 %D 2006 %I %X Molecular biology techniques of Fish, PCR-DGGE and PCR-Cloning-Sequencing were used to qualitatively and quantitatively analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in SBR large pilot-scale plant, UASB-A/O lab-scale reactor, A/O pilot-scale plant and SBR lab-scale reactor with the performance of short-cut nitrification-denitrification treating actual wastewater. Fish results showed that in the above 4 processes, AOB became dominant with compared to NOB and accounted for 3%~12% of total biomass. No NOB was detected in both SBR pilot-scale and lab-scale reactors. A very small amount of Nitrospira and Nitrobacteria below 0.2% was found in A/O pilot-scale plant and UASB-A/O lab-scale reactor, respectively. PCR-DGGE results showed that the AOB in SBR pilot-scale, A/O pilot-scale and UASB-A/O lab-scale processes were phylogenetically related to Nitrosomonas-like species with a melting point in the range 30% to 50%. PCR-Cloning-Sequencing results showed that all clones from the sludge sample of the SBR large pilot-scale plant were affiliated with Nitrosomonas and 60% of the clones were affiliated with Nitrosomonas europaea. %K short-cut nitrification-denitrification %K AOB %K FISH %K PCR-DGGE %K PCR-Cloning-Sequencing
短程脱氮 %K AOB %K FISH %K PCR-DGGE %K PCR-Cloning-Sequencmg %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=03A55E61A8750ACAC6AF81EF9E2AC838&aid=169433C2CBFFC52D&yid=37904DC365DD7266&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=7F9B7E84827A650F&eid=547991B346A6585E&journal_id=0253-2468&journal_name=环境科学学报&referenced_num=0&reference_num=10