EFFECTS OF CADMIUM ON THE CONJUGATION OF RAT KIDNEY CORTEX
镉对大鼠肾皮质结合反应的影响

The effects of cadmium on two kinds of conjugation reaction in rat kidney cortex in vivo and in vitro were studied. One was kidney cortex microsomal glutathione S-transferase (GST), and the other was glucuronic acid(GA)conjugation in isolated kidney cortex cells. It was found: l). Cadmium could inhibit kidney cortex microsomal GST activities in vitro under Cd2+ concentration from 107mol/L to 103mol/L;2). GST activity was " not markedly changed in vivo after the ip injection of cadmium(2.4mg(kg·d)-l for 3 days); 3). When CdCl2 was administered to rats in vivo by ip injection in the same way it could markedly decrease the conjugation of glucuronic acid with 7-hydroxycoumarin in isolated rat kidney cortex cells; and 4).Lower Cd2+concentration (10-7mol/L)could increase the conjugation of GA with 7-HC in isolated rat kidney cortex cells in vitro, but higher Cd2+concentrations(10-6mol/L-10-2mol/L)could markedly decrease the conjugation of GA with 7-HC in vitro.