Analysis of Bacterial Community in the Polluted Water of Dianchi Lake by Using T-RFLP Technique
应用T-RFLP技术分析滇池污染水体的细菌群落

In order to assess the dominant microbial communities in the polluted water of the Dianchi Lake, terminal restriction fragment length polymorphism (T-RFLP) technique was used to analyze the characteristics and diversity of bacterial communities in nine sites. Clustering analysis based on the T-RFLP profiles showed that the bacterial community structures varies considerably, which may be caused by the pollutant variation at different geographic locations. According to the further analysis of T-RFLP profiles with clone library technique, the dominant bacteria groups in nine sites represented by distinctive terminal restriction fragments (T-RFs) were identified as genera of Pseudomonas, Aphanizomenon and Rhodobacter.