Rapid Method to Extract High-Quality RNA from Activated Sludge
活性污泥高质量RNA快速提取方法研究

An effective and fast RNA isolation method of activated sludge was established and five different methods were compared based on RNA yield,purity,integrity,RT-PCR amplification of 16S rRNA genes and subsequent terminal restriction fragment length polymorphism(T-RFLP) analysis.That is,the precipitated activated sludge was washed with TENP and PBS buffer,followed by using lysozyme and TRIzol to direct lysis of microbial cells,chloroform to remove protein and most of the DNA from bacterial lysate,isopropanol t...