%0 Journal Article
%T Rapid Method to Extract High-Quality RNA from Activated Sludge<br>活性污泥高质量RNA快速提取方法研究
%A JIN Min
%A ZHAO Zu-guo
%A QIU Zhi-gang
%A WANG Jing-feng
%A CHEN Zhao-li
%A SHEN Zhi-qiang
%A LI Chao
%A WANG Xin-wei
%A DONG Yan
%A LI Jun-wen
%A <br>金敏
%A 赵祖国
%A 邱志刚
%A 王景峰
%A 陈照立
%A 谌志强
%A 李超
%A 王新为
%A 董彦
%A 李君文
%J 环境科学
%D 2010
%I 
%X An effective and fast RNA isolation method of activated sludge was established and five different methods were compared based on RNA yield,purity,integrity,RT-PCR amplification of 16S rRNA genes and subsequent terminal restriction fragment length polymorphism(T-RFLP) analysis.That is,the precipitated activated sludge was washed with TENP and PBS buffer,followed by using lysozyme and TRIzol to direct lysis of microbial cells,chloroform to remove protein and most of the DNA from bacterial lysate,isopropanol t...
%K activated sludge
%K RNA
%K bacterium community
%K extraction
%K T-RFLP<br>活性污泥
%K RNA
%K 菌群
%K 提取
%K T-RFLP
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=6A6116636A068E1CF86B462B2AD44804&yid=140ECF96957D60B2&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=89AC6B0ADBEA2741&eid=FDC7AF55F77D8CD4&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=6