Integration and Expression of Polyphosphate Kinase Gene in Pseudomonas putida
聚磷激酶基因在假单胞菌中的整合和表达

In order to construct high accumulating-phosphate microorganism,the ppk gene from E.coli was inserted to the broad-host-range plasmid pBBR1MCS-2 to form plasmid pBBR1MCS-2-ppk.The complete ppk gene with promoter and terminator sequences from pBBR1MCS-2-ppk was then cloned and inserted to suicide plasmid pUTmini-Tn5 to form plasmid pUTmini-Tn5-ppk,which was transformed into Pseudomonas putida KT2440 by triparental conjugation.Finally,ppk gene was integrated into the chromosomal DNA of KT2440.The results of R...