%0 Journal Article
%T Integration and Expression of Polyphosphate Kinase Gene in Pseudomonas putida<br>聚磷激酶基因在假单胞菌中的整合和表达
%A DU Hong-wei
%A WU Jun
%A XIAO Lin
%A YANG Liu-yan
%A JIANG Li-juan
%A WANG Xiao-lin
%A <br>杜宏伟
%A 武俊
%A 肖琳
%A 杨柳燕
%A 蒋丽娟
%A 王晓琳
%J 环境科学
%D 2009
%I 
%X In order to construct high accumulating-phosphate microorganism,the ppk gene from E.coli was inserted to the broad-host-range plasmid pBBR1MCS-2 to form plasmid pBBR1MCS-2-ppk.The complete ppk gene with promoter and terminator sequences from pBBR1MCS-2-ppk was then cloned and inserted to suicide plasmid pUTmini-Tn5 to form plasmid pUTmini-Tn5-ppk,which was transformed into Pseudomonas putida KT2440 by triparental conjugation.Finally,ppk gene was integrated into the chromosomal DNA of KT2440.The results of R...
%K poly-phosphate
%K poly-phosphate kinase (PPK)
%K triparetal conjugation
%K Pseudomonas putida<br>聚磷
%K 聚磷激酶（PPK）
%K 三亲接合
%K 恶臭假单胞菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=07ACDCCC69AFFB95236928405E6B7282&yid=DE12191FBD62783C&vid=340AC2BF8E7AB4FD&iid=F3090AE9B60B7ED1&sid=1BC7431C81AD2093&eid=05F456AEC361A0E3&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=26