Immobilization of Crude Laccase onto Anion Exchange Resin and Its Application in Decoloration of Malachite Green
树脂D201上粗漆酶的固定化及对孔雀石绿的脱色

Crude laccase from Trametes versicolor was immobilized onto anion exchange resin D201 by three methods,i.e.,direct electrostatic adsorption(D201-Lac-Ⅰ),crosslinking after electrostatic adsorption(D201-Lac-Ⅱ) and electrostatic adsorption after treating D201 with glutaraldehyde(D201-Lac-Ⅲ).Compared to direct electrostatic adsorption,the immobilized laccase amount of D201-Lac-Ⅱ increased by 4.65 times but the laccase activity was decreased to 4.8%,while the laccase activity on D201-Lac-Ⅲ increased by 2.99 times,with the immobilization amount decreased to 51%.Shadows of laccase aggregation on D201-Lac-Ⅲ were found by transmission electron microscopy(TEM).Continuous batch decoloration of malachite green demonstrated that the decoloration efficiency of D201-Lac-Ⅲ remained in the range of 40% to 55% for more than 210 hours,in addition,the enzyme activity on D201-Lac-Ⅲ maintained unchanged while the activity of free laccase declined to less than 20% under the same condition.All of the results above indicated that D201-Lac-Ⅲ had a significantly enhanced stability and good reusability.Considering the low price and simple production procedure of crude laccase,D201-Lac-Ⅲ could be promising for water treatment purpose.