%0 Journal Article
%T Immobilization of Crude Laccase onto Anion Exchange Resin and Its Application in Decoloration of Malachite Green
树脂D201上粗漆酶的固定化及对孔雀石绿的脱色
%A QI Xu-liang
%A LIU Xiang
%A LIU Bo
%A WANG Lin
%A WANG Xiao-chun
%A FANG Chao
%A
戚绪亮
%A 刘翔
%A 刘波
%A 王林
%A 王小春
%A 方超
%J 环境科学
%D 2012
%I
%X Crude laccase from Trametes versicolor was immobilized onto anion exchange resin D201 by three methods,i.e.,direct electrostatic adsorption(D201-Lac-Ⅰ),crosslinking after electrostatic adsorption(D201-Lac-Ⅱ) and electrostatic adsorption after treating D201 with glutaraldehyde(D201-Lac-Ⅲ).Compared to direct electrostatic adsorption,the immobilized laccase amount of D201-Lac-Ⅱ increased by 4.65 times but the laccase activity was decreased to 4.8%,while the laccase activity on D201-Lac-Ⅲ increased by 2.99 times,with the immobilization amount decreased to 51%.Shadows of laccase aggregation on D201-Lac-Ⅲ were found by transmission electron microscopy(TEM).Continuous batch decoloration of malachite green demonstrated that the decoloration efficiency of D201-Lac-Ⅲ remained in the range of 40% to 55% for more than 210 hours,in addition,the enzyme activity on D201-Lac-Ⅲ maintained unchanged while the activity of free laccase declined to less than 20% under the same condition.All of the results above indicated that D201-Lac-Ⅲ had a significantly enhanced stability and good reusability.Considering the low price and simple production procedure of crude laccase,D201-Lac-Ⅲ could be promising for water treatment purpose.
%K crude laccase
%K anion exchange resin
%K immolization
%K stability
%K dye decoloration
粗漆酶
%K 阴离子树脂
%K 固定化
%K 稳定性
%K 脱色
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=669A8D56CCC2B6A1DF8746BBA489DA14&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=5D311CA918CA9A03&sid=B765C19BA0C80620&eid=43D114DF111A2096&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=26