Effects of pH and CU~(2+) on the Detection of the Microcystin-LR Based on Fluorescence Immunoassay and Their Elimination
pH和铜离子对微囊藻毒素-LR荧光免疫检测的影响与消除

The effects of pH and Cu 2+ on the detection of the Microcystin-LR based on evanescent wave all-fiber immunosensor (EWAI) and their elimination were studied. The experimental results indicated that both low pH and high pH greatly affected the immunoassay results of MC-LR. When pH was less than 6 or higher than 8,the fluorescence signals detected by immunosensor system obviously reduced with the decrease or increase of pH. While pH ranged from 6 to 8,IC 50 and the linear working range of MC-LR calculated fro...