One Step Time-Resolved Fluorescence Immunoassay for Direct Estimation of Microcystins
时间分辨荧光免疫一步法检测微囊藻毒素的研究

A direct-competitive time-resolved fluorescence immunoassay(TRFIA) for microcystin detection was established,which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG.The optimal dilution of europium labeled MCLR-BSA conjugant is(1/50) and most appropriate titration of anti-microcystin-LR(MCLR) monoclonal antibodies is 100 ng/mL.The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05(ng/mL) to 10(ng/mL) The detection limit of the method is 0.02(ng/mL) for MCLR,recoveries for microcystin(>94%) in quantitative range is satisfactory.