%0 Journal Article
%T One Step Time-Resolved Fluorescence Immunoassay for Direct Estimation of Microcystins<br>时间分辨荧光免疫一步法检测微囊藻毒素的研究
%A LEI La-mei
%A HAN Bo-ping
%A SONG Li-rong
%A <br>雷腊梅
%A 韩博平
%A 宋立荣
%J 环境科学
%D 2007
%I 
%X A direct-competitive time-resolved fluorescence immunoassay(TRFIA) for microcystin detection was established,which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG.The optimal dilution of europium labeled MCLR-BSA conjugant is(1/50) and most appropriate titration of anti-microcystin-LR(MCLR) monoclonal antibodies is 100 ng/mL.The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05(ng/mL) to 10(ng/mL) The detection limit of the method is 0.02(ng/mL) for MCLR,recoveries for microcystin(>94%) in quantitative range is satisfactory.
%K microcystin
%K time-resolved fluorescence immunoassay
%K detection<br>微囊藻毒素
%K 时间分辨荧光免疫分析
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=BC85E858628321E107C50DE884B40288&yid=A732AF04DDA03BB3&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=11632AEF1E1F2092&eid=4AA5FA7F666BDD0A&journal_id=0250-3301&journal_name=环境科学&referenced_num=1&reference_num=12