Localization of Degradation Enzyme in Pseudomonas sp. for an Aromatic Sulfur-Containing Compound and Identification of Products in Cells
含硫芳香族化合物降解酶的定域及胞内产物的鉴定

In order to study the biodegradation way of an aromatic sulfurcontaining compound,the resting cell technique was used to get different enzyme.The experimental results show that the degradation enzyme for 4-NO2-Ph-SO2-CH2-CO-OC4H9-i mainly locates in cell and on the membrane.The enzyme in cells converts the tested compound to 4-NO2Ph-SO3H with bond cleavage between carbon and sulfur.By the enzyme located on the membrane the compound is degraded as 4-NO2Ph-SO3H and 4-NO2Ph-SO2-CH2-COOH.The later is yielded with the bond cleavage between carbonyl and alkoxy group.