%0 Journal Article %T Localization of Degradation Enzyme in Pseudomonas sp. for an Aromatic Sulfur-Containing Compound and Identification of Products in Cells
含硫芳香族化合物降解酶的定域及胞内产物的鉴定 %A Zhang Chengdong %A Zhang Aiqian %A Han Shuokui %A Wang Liansheng %A
张承东 %A 张爱茜 %A 韩朔睽 %A 王连生 %J 环境科学 %D 2000 %I %X In order to study the biodegradation way of an aromatic sulfurcontaining compound,the resting cell technique was used to get different enzyme.The experimental results show that the degradation enzyme for 4-NO2-Ph-SO2-CH2-CO-OC4H9-i mainly locates in cell and on the membrane.The enzyme in cells converts the tested compound to 4-NO2Ph-SO3H with bond cleavage between carbon and sulfur.By the enzyme located on the membrane the compound is degraded as 4-NO2Ph-SO3H and 4-NO2Ph-SO2-CH2-COOH.The later is yielded with the bond cleavage between carbonyl and alkoxy group. %K pnitrophenylsulfonyl isobutyl acetate %K resting cell technique %K localization of degradation enzyme %K products in cells
苯砜基乙酸异丁酯 %K 静息细胞技术 %K 降解酶的定域 %K 胞内产物 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=E6F03E143BD20E72&yid=9806D0D4EAA9BED3&vid=659D3B06EBF534A7&iid=E158A972A605785F&sid=869807E2D7BED9EC&eid=39EEF47180459690&journal_id=0250-3301&journal_name=环境科学&referenced_num=4&reference_num=8