Hydrolysis and Mechanism of Wastewater Contained Protein Using Immobilized Protein Enzyme
固定化蛋白酶对废水中蛋白质的水解效果与机理

Protein-enzyme was immobilized on big-hole resin,activated charcoal and silica particles by a absorption method.This study focuses on effect of enzyme immobilization,thermal stability and hydrolysis efficiency of wastewater.The result of batch test show that the ratio of immobilized enzyme on activated charcoal,big-hole resin and silica particles was 61.95%,37.85% and 20.31% respectively.The highest of thermal ability of activated charcoal,big-hole resin and silica particles was 18.68mg/min,21.48mg/min and 3.6mg/min respectively at pH 5.5.The total amino-acids of big-hole resin and activated charcoal immobilized enzyme after hydrolysis was respectively increased to 21.26 times and 14.6 times. The kind of amino-acid was increased from 14 to 16 kinds. The continuous-flow test result indicate that concentration of substrate in influent had significant effect on the hydrolysis rate of immobilized enzyme reactor.The reactor was able to gain hydrolysis rate of 80% for protein and 36.1%(VFA/COD%) for organic materials in the wastewater with a moderate concentration of protein (500mg/L).The ethanol and acetic of volatile hydrolysis-mixture composition were 50.3% and 33.3% respectively.