%0 Journal Article
%T Hydrolysis and Mechanism of Wastewater Contained Protein Using Immobilized Protein Enzyme<br>固定化蛋白酶对废水中蛋白质的水解效果与机理
%A YU Hong-bing
%A LIN Xue-yu
%A ZHANG Lan-ying
%A ZHANG Lei
%A YANG Xue-mei
%A LIU Ji-li
%A <br>于宏兵
%A 林学钰
%A 张兰英
%A 张蕾
%A 杨雪梅
%A 刘继莉
%J 环境科学
%D 2005
%I 
%X Protein-enzyme was immobilized on big-hole resin,activated charcoal and silica particles by a absorption method.This study focuses on effect of enzyme immobilization,thermal stability and hydrolysis efficiency of wastewater.The result of batch test show that the ratio of immobilized enzyme on activated charcoal,big-hole resin and silica particles was 61.95%,37.85% and 20.31% respectively.The highest of thermal ability of activated charcoal,big-hole resin and silica particles was 18.68mg/min,21.48mg/min and 3.6mg/min respectively at pH 5.5.The total amino-acids of big-hole resin and activated charcoal immobilized enzyme after hydrolysis was respectively increased to 21.26 times and 14.6 times. The kind of amino-acid was increased from 14 to 16 kinds. The continuous-flow test result indicate that concentration of substrate in influent had significant effect on the hydrolysis rate of immobilized enzyme reactor.The reactor was able to gain hydrolysis rate of 80% for protein and 36.1%(VFA/COD%) for organic materials in the wastewater with a moderate concentration of protein (500mg/L).The ethanol and acetic of volatile hydrolysis-mixture composition were 50.3% and 33.3% respectively.
%K immobilizing
%K protein enzyme
%K hydrolysis
%K wastewater<br>固定化
%K 蛋白酶
%K 水解
%K 废水
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=64CD0AA99DD39F69401C615B85F123EF&aid=84C079E5066C7FD3&yid=2DD7160C83D0ACED&vid=96C778EE049EE47D&iid=38B194292C032A66&sid=4BB057F167CF3A60&eid=7555FB9CC973F695&journal_id=0250-3301&journal_name=环境科学&referenced_num=0&reference_num=11