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海洋与湖沼 2009
GENOMIC DNA EXTRACTION AND RAPD ANALYSIS ON HYRIOPSIS CUMINGII DISTINGUISHED BY PEARL-BREED
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Abstract:
Random amplified polymorphic DNA(RAPD) technique was applied to assess the genetic diversity of two populations of Hyriopsis cumingii distinguished by pearl-breed,six individuals from each population were randomly selected,followed by genomic DNA extraction using the SDS-phenol-chloroform method and KIT method.Of 80 10-nucleotide-long random primers used in the preliminary analysis,12 primers produced well-amplified and reproducible band patterns.They were screened and used for further analysis.The amplifie...