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GENOMIC DNA EXTRACTION AND RAPD ANALYSIS ON HYRIOPSIS CUMINGII DISTINGUISHED BY PEARL-BREED
人工育珠对三角帆蚌(Hyriopsis cumingii)遗传基因影响的初步研究

Keywords: Hyriopsis cumingii,Artificial pearl-breading,Random amplified polymorphic DNA (RAPD),Genetic diversity
三角帆蚌
,人工育珠,随机扩增多态性DNA(RAPD),遗传多样性

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Abstract:

Random amplified polymorphic DNA(RAPD) technique was applied to assess the genetic diversity of two populations of Hyriopsis cumingii distinguished by pearl-breed,six individuals from each population were randomly selected,followed by genomic DNA extraction using the SDS-phenol-chloroform method and KIT method.Of 80 10-nucleotide-long random primers used in the preliminary analysis,12 primers produced well-amplified and reproducible band patterns.They were screened and used for further analysis.The amplifie...

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