%0 Journal Article %T GENOMIC DNA EXTRACTION AND RAPD ANALYSIS ON HYRIOPSIS CUMINGII DISTINGUISHED BY PEARL-BREED
人工育珠对三角帆蚌(Hyriopsis cumingii)遗传基因影响的初步研究 %A YANG Pin-Hong %A WANG Zhi-Tao %A
杨品红 %A 王志陶 %J 海洋与湖沼 %D 2009 %I %X Random amplified polymorphic DNA(RAPD) technique was applied to assess the genetic diversity of two populations of Hyriopsis cumingii distinguished by pearl-breed,six individuals from each population were randomly selected,followed by genomic DNA extraction using the SDS-phenol-chloroform method and KIT method.Of 80 10-nucleotide-long random primers used in the preliminary analysis,12 primers produced well-amplified and reproducible band patterns.They were screened and used for further analysis.The amplifie... %K Hyriopsis cumingii %K Artificial pearl-breading %K Random amplified polymorphic DNA (RAPD) %K Genetic diversity
三角帆蚌 %K 人工育珠 %K 随机扩增多态性DNA(RAPD) %K 遗传多样性 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=57D1FDBDFB223A890A80F625D45BD690&yid=DE12191FBD62783C&vid=1371F55DA51B6E64&iid=94C357A881DFC066&sid=06F643376BC2509E&eid=46C2A519EDDA03DD&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=33