DEVELOPMENT OF 17 SINGLE NUCLEOTIDE POLYMORPHISMS (SNPs) IN CRASSOSTREA GIGAS SEARCHED FROM EST DATABASE
长牡蛎(Crassostrea gigas)17 个EST-SNP标记的开发

Some 29000 EST sequences of Crassostrea gigas from the GenBank were clustered into 4548 groups, of which 1079 contained 4 or more ESTs. After manual quality filtering, 313 clusters could be used for the SNP development. 82 candidate SNPs were amplified with fragment length discrepant allele specific PCR (FLDAS-PCR), of which 17 were polymorphic in a wild population. The function of the ESTs was predicted, and ORF was deduced based on BlastX analysis. The expected and observed heterozygosities of the SNPs ra...