%0 Journal Article
%T DEVELOPMENT OF 17 SINGLE NUCLEOTIDE POLYMORPHISMS (SNPs) IN CRASSOSTREA GIGAS SEARCHED FROM EST DATABASE<br>长牡蛎(Crassostrea gigas)17 个EST-SNP标记的开发
%A WANG Shao-Zong
%A LI Li
%A QI Hai-Gang
%A ZHANG Guo-Fan
%A <br>王绍宗
%A 李莉
%A 亓海刚
%A 张国范
%J 海洋与湖沼
%D 2010
%I 
%X Some 29000 EST sequences of Crassostrea gigas from the GenBank were clustered into 4548 groups, of which 1079 contained 4 or more ESTs. After manual quality filtering, 313 clusters could be used for the SNP development. 82 candidate SNPs were amplified with fragment length discrepant allele specific PCR (FLDAS-PCR), of which 17 were polymorphic in a wild population. The function of the ESTs was predicted, and ORF was deduced based on BlastX analysis. The expected and observed heterozygosities of the SNPs ra...
%K Crassostrea gigas
%K Single nucleotide polymorphism (SNP)
%K Expressed sequence tag (EST)
%K Fragment length discrepant allele specific PCR (FLDAS-PCR)<br>长牡蛎
%K 单核苷酸多态性
%K 表达序列标签
%K 片段长度差异等位基因特异性PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=77004FB3262B6CE41BD729F4553066CA&yid=140ECF96957D60B2&vid=2001E0D53B7B80EC&iid=0B39A22176CE99FB&sid=6826CBE9C80ACB20&eid=C1B34927D429E92F&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=6