DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)
应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)

Fish viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus in fish causing serious fish mortality. With the results of genomes sequence analysis on VHSV, two pairs of primers were designed. Under optimized RT-PCR conditions, target regions of VHSV glycoprotein gene were amplified using VHS1-2 and VHS3-4 primers. Products of 279bp segment were shown after the nested RT-PCR reaction. Negative control showed no amplification. The RT-PCR product was proved identical to previously reported VHSV sequence. The detection limit of the nested RT-PCR assay is 0.1pg. Therefore, the nested RT-PCR assay, a sensitive, specific, and quick tool for VHSV detection is established.