%0 Journal Article
%T DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)<br>应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)
%A NI Sui
%A YU Xiao-Wei
%A WANG Jian-Ping
%A LEI Ai-Ying
%A ZENG Di-Gang
%A WU Xiong-Fei
%A <br>倪穗
%A 余晓巍
%A 王建平
%A 雷爱莹
%A 曾地刚
%A 吴雄飞
%J 海洋与湖沼
%D 2009
%I 
%X Fish viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus in fish causing serious fish mortality. With the results of genomes sequence analysis on VHSV, two pairs of primers were designed. Under optimized RT-PCR conditions, target regions of VHSV glycoprotein gene were amplified using VHS1-2 and VHS3-4 primers. Products of 279bp segment were shown after the nested RT-PCR reaction. Negative control showed no amplification. The RT-PCR product was proved identical to previously reported VHSV sequence. The detection limit of the nested RT-PCR assay is 0.1pg. Therefore, the nested RT-PCR assay, a sensitive, specific, and quick tool for VHSV detection is established.
%K 病毒性出血性败血症病毒
%K 巢式逆转录聚合酶链反应
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=3D5978994498DDB9C237CEBC17952C61&yid=DE12191FBD62783C&vid=1371F55DA51B6E64&iid=E158A972A605785F&sid=F176272DD933A0BB&eid=8C267C8DC97FEEEF&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=10