SCREENING, IDENTIFICATION OF ANTARCTIC COLD-ADAPTED PETROLEUM HYDROCARBON-DEGRADING BACTERIA AND LOW-TEMPERATURE DEGRADING ADAPTATION ANALYSIS
南极低温降解菌的筛选、鉴定与低温降解适应性分析

Low temperature microorganism resources of Antarctica were abundant and the scholar abroad have focused on obtaining cold-adapted degrading bacteria. The method of combination 2216E with MMC culture was used for screening petroleum hydrocarbon-degrading cold-adapted bacteria which were from Antarctic seawaters . Degradation efficient, molecular identification of 16S rRNA amplified from four Antarctic cold-adapted degrading bacteria and phylogenetic tree based on 16S rRNA gene was carried. Antarctic cold-adapted degrading bacteria NJ49 was chosen to study cold adaptation. The results suggested that three marine bacteria cold-adapted bacteria (namely NJ41, NJ49, NJ289) grew well in the culture which diesel was the sole carbon source and energy source. Results of 16S rRNA identification revealed that Antarctic cold-adapted degrading bacteria NJ41, NJ49 and NJ289 belonged to the described genus Planococcus, Shewanella, Pseudo-alteromonas, respectively. Data of fatty acid change revealed that the Antarctic cold-adapted degrading bacteria NJ49 maybe adapt cold condition to degrade by membrane modification of changing fatty acid.