%0 Journal Article %T SCREENING, IDENTIFICATION OF ANTARCTIC COLD-ADAPTED PETROLEUM HYDROCARBON-DEGRADING BACTERIA AND LOW-TEMPERATURE DEGRADING ADAPTATION ANALYSIS
南极低温降解菌的筛选、鉴定与低温降解适应性分析 %A LIU Fang-Ming %A MIAO Jin-Lai %A DONG Chun-Xia %A WANG Yi-Bin %A ZANG Jia-Ye %A
刘芳明 %A 缪锦来 %A 董春霞 %A 王以斌 %A 臧家业 %J 海洋与湖沼 %D 2010 %I %X Low temperature microorganism resources of Antarctica were abundant and the scholar abroad have focused on obtaining cold-adapted degrading bacteria. The method of combination 2216E with MMC culture was used for screening petroleum hydrocarbon-degrading cold-adapted bacteria which were from Antarctic seawaters . Degradation efficient, molecular identification of 16S rRNA amplified from four Antarctic cold-adapted degrading bacteria and phylogenetic tree based on 16S rRNA gene was carried. Antarctic cold-adapted degrading bacteria NJ49 was chosen to study cold adaptation. The results suggested that three marine bacteria cold-adapted bacteria (namely NJ41, NJ49, NJ289) grew well in the culture which diesel was the sole carbon source and energy source. Results of 16S rRNA identification revealed that Antarctic cold-adapted degrading bacteria NJ41, NJ49 and NJ289 belonged to the described genus Planococcus, Shewanella, Pseudo-alteromonas, respectively. Data of fatty acid change revealed that the Antarctic cold-adapted degrading bacteria NJ49 maybe adapt cold condition to degrade by membrane modification of changing fatty acid. %K tarctic sea %K degrading bacteria %K Diesel %K 16S rRNA %K Low-temperature adaptation
极海洋 %K 降解菌 %K 柴油 %K 16S %K rRNA %K 低温适应性 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=10F18692CD69EACA2DF7FC109993D35C&aid=FEB0E218CB31012920A563233B393E5D&yid=140ECF96957D60B2&vid=2001E0D53B7B80EC&iid=94C357A881DFC066&sid=15F713CDE16A589C&eid=780091CB32840698&journal_id=0029-814X&journal_name=海洋与湖沼&referenced_num=0&reference_num=0