Fed-batch Cultivation of Protocorm-like Bodies of Dendrobium huoshanense for Producing Polysaccharides in an Air-lift Bioreactor
霍山石斛类原球茎在气升式反应器中补料培养合成多糖

The cultivation of protocorm-like bodies of Dendrbium huoshanense was carried out in a 10 L air-lift bioreactor.The effects of inoculum size,aeration rate,carbon,phosphate sources on the cell growth and accumulation of polysaccharides were investigated in suspension cultures of protocorm-like bodies from Dendrobium huoshanense.Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L(fresh weight)cells and aeration rate of 0.5 L/min.The optimal concentrations of sucrose and phosphate in the medium for the cell growth were 30 g/L and 2.5 mmol/L,respectively.A total of 0.312 mmol/L of medium phosphate was better for the accumulation of polysaccharides.The results in two-step cultivation indicated that the maximum biomass and polysaccharides yield were 46.7 g/L(dry weight)and 8.15 g/L,which are 1.5 and 2.6 fold of those in one-step cultivation respectively.