%0 Journal Article
%T Fed-batch Cultivation of Protocorm-like Bodies of Dendrobium huoshanense for Producing Polysaccharides in an Air-lift Bioreactor<br>霍山石斛类原球茎在气升式反应器中补料培养合成多糖
%A WEI Ming
%A JIANG Shao-tong
%A LUO Jian-ping
%A <br>魏明
%A 姜绍通
%A 罗建平
%J 过程工程学报
%D 2007
%I 
%X The cultivation of protocorm-like bodies of Dendrbium huoshanense was carried out in a 10 L air-lift bioreactor.The effects of inoculum size,aeration rate,carbon,phosphate sources on the cell growth and accumulation of polysaccharides were investigated in suspension cultures of protocorm-like bodies from Dendrobium huoshanense.Thirty-day-old cells were transferred into liquid medium with the inoculum density of 100 g/L(fresh weight)cells and aeration rate of 0.5 L/min.The optimal concentrations of sucrose and phosphate in the medium for the cell growth were 30 g/L and 2.5 mmol/L,respectively.A total of 0.312 mmol/L of medium phosphate was better for the accumulation of polysaccharides.The results in two-step cultivation indicated that the maximum biomass and polysaccharides yield were 46.7 g/L(dry weight)and 8.15 g/L,which are 1.5 and 2.6 fold of those in one-step cultivation respectively.
%K Dendrobium huoshanense
%K protocorm-like bodies
%K air-lift bioreactor
%K polysaccharides
%K fed-batch cultivation<br>霍山石斛
%K 类原球茎
%K 气升式反应器
%K 多糖
%K 补料培养
%K 霍山石斛
%K 类原球茎
%K 气升式
%K 反应器
%K 补料培养
%K 多糖合成
%K 多糖积累
%K Bioreactor
%K Polysaccharides
%K Producing
%K 糖产率
%K 生物量
%K 结果
%K 时间
%K 最佳
%K 策略
%K 培养方式
%K 力学特性
%K 磷酸盐
%K 蔗糖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=FE09EDBDBB744C0F2CB4485AAE1CACBB&yid=A732AF04DDA03BB3&vid=DF92D298D3FF1E6E&iid=0B39A22176CE99FB&sid=315A3D008C6ECFC8&eid=9EF602EA28138BEA&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=15