Extraction and Characterization of Serotransferrin from Monopterus albus
黄鳝血清转铁蛋白的提取及表征

The crude extraction of serum protein in Monopterus albus obtained by salting was purified by ion exchange chromatography.By means of biological mass spectrometry,the protein was identified as serotransferrin,which was the serum transferrin.Its molecular weight,surface morphology and secondary structure were characterized by SDS-PAGE,atomic force microscopy and circular dichroism,respectively.The results show that the molecular weight of serotransferrin is 74 kDa,its content 0.750 mg/mL,including α-helix 29.8% and α-fold 6.6%.