%0 Journal Article
%T Extraction and Characterization of Serotransferrin from Monopterus albus<br>黄鳝血清转铁蛋白的提取及表征
%A WANG Bo-wen
%A REN Tian-rui
%A DONG Ya-ming
%A WANG Feng
%A <br>王博文
%A 任天瑞
%A 董亚明
%A 王丰
%J 过程工程学报
%D 2012
%I 
%X The crude extraction of serum protein in Monopterus albus obtained by salting was purified by ion exchange chromatography.By means of biological mass spectrometry,the protein was identified as serotransferrin,which was the serum transferrin.Its molecular weight,surface morphology and secondary structure were characterized by SDS-PAGE,atomic force microscopy and circular dichroism,respectively.The results show that the molecular weight of serotransferrin is 74 kDa,its content 0.750 mg/mL,including α-helix 29.8% and α-fold 6.6%.
%K Monopterus albus
%K transferrin
%K SDS-PAGE
%K biological mass spectrum
%K atomic force microscopy<br>黄鳝
%K 转铁蛋白
%K 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳
%K 生物质谱
%K 原子力显微镜
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=28538CA98C300078BC2E657AECDBDDF1&yid=99E9153A83D4CB11&vid=59906B3B2830C2C5&iid=CA4FD0336C81A37A&sid=F122871CC7EC92DC&eid=B47A0E731AF43EB2&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=0