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过程工程学报 2009
Effect of Cycloheximide on Regulation of Metabolic Pathway for L-Malic Acid Accumulation by Rhizopus oryzae
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Abstract:
The effects of fumarase inhibitor cycloheximide on fermentation of L-malic acid and fumaric acid by Rhizopus oryzae ME-M15 were investigated.The results showed that the addition of cycloheximide(15 mg/L) during the acid production stage after 16 h incubation greatly affected the metabolism of Rhizopus oryzae ME-M15.The activity of cytosolic isoenzyme of fumarase was decreased markedly by 48.7%, but the activity of NAD-malate dehydrogenase in the cytosol fraction did not change significantly in the presence ...
