%0 Journal Article %T Effect of Cycloheximide on Regulation of Metabolic Pathway for L-Malic Acid Accumulation by Rhizopus oryzae
放线菌酮对米根霉积累L-苹果酸代谢途径的调控作用 %A HE Hao %A LI Shuang %A XU Qing %A ZHANG Kai %A HUANG He %A
何皓 %A 徐晴 %A 李霜 %A 张凯 %A 黄和 %J 过程工程学报 %D 2009 %I %X The effects of fumarase inhibitor cycloheximide on fermentation of L-malic acid and fumaric acid by Rhizopus oryzae ME-M15 were investigated.The results showed that the addition of cycloheximide(15 mg/L) during the acid production stage after 16 h incubation greatly affected the metabolism of Rhizopus oryzae ME-M15.The activity of cytosolic isoenzyme of fumarase was decreased markedly by 48.7%, but the activity of NAD-malate dehydrogenase in the cytosol fraction did not change significantly in the presence ... %K L-malic acid %K Rhizopus oryzae %K cycloheximide %K fumarase %K fumaric acid
L-苹果酸 %K 米根霉 %K 放线菌酮 %K 富马酸酶 %K 富马酸 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=4C37CEB8C62DB590712D9E0D3003CC3D&yid=DE12191FBD62783C&vid=9CF7A0430CBB2DFD&iid=CA4FD0336C81A37A&sid=D59111839E7C8BDF&eid=3F0AF5EDBC960DB0&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=14