Immobilization of Lipase on PVDF-SiO2 Composite Microspheres
在PVDF-SiO2复合微球上的脂肪酶固定化

Polyvinylidene fluoride(PVDF)-SiO2 composite microspheres enveloping SiO2 were prepared by phase transition process and used as carrier to immobilize Candida rugosa lipase(CRL).It is found that CRL immobilized on PVDF or PVDF-SiO2 composite microspheres has a large loading.As to PVDF-SiO2 composite microspheres,with the increase of SiO2 amount,both the loading and recovery rate of activity increase.When the mass ratio of PVDF to SiO2 is 1:1,they have the maximal loading of 200 mg/g and the maximal recovery rate of activity of 50.98%,the PVDF-SiO2 composite microspheres are the optimized carrier in the experiment.Comparing PVDF-SiO2 composite with SiO2,the former is superior in filtration and has no pollution on substrate solution.