%0 Journal Article
%T Immobilization of Lipase on PVDF-SiO2 Composite Microspheres<br>在PVDF-SiO2复合微球上的脂肪酶固定化
%A TAN Yan-li
%A WANG Yu-jun
%A SUN Hai-tao
%A LUO Guang-sheng
%A <br>谭艳丽
%A 王玉军
%A 孙海涛
%A 骆广生
%J 过程工程学报
%D 2012
%I 
%X Polyvinylidene fluoride(PVDF)-SiO2 composite microspheres enveloping SiO2 were prepared by phase transition process and used as carrier to immobilize Candida rugosa lipase(CRL).It is found that CRL immobilized on PVDF or PVDF-SiO2 composite microspheres has a large loading.As to PVDF-SiO2 composite microspheres,with the increase of SiO2 amount,both the loading and recovery rate of activity increase.When the mass ratio of PVDF to SiO2 is 1:1,they have the maximal loading of 200 mg/g and the maximal recovery rate of activity of 50.98%,the PVDF-SiO2 composite microspheres are the optimized carrier in the experiment.Comparing PVDF-SiO2 composite with SiO2,the former is superior in filtration and has no pollution on substrate solution.
%K polyvinylidene fluoride
%K silica
%K immobilized enzyme
%K organic and inorganic composite microspheres<br>聚偏氟乙烯
%K 二氧化硅
%K 固定化酶
%K 无机有机复合微球
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=ACE46FDFF1576C24FCD7387309EDA016&yid=99E9153A83D4CB11&vid=59906B3B2830C2C5&iid=38B194292C032A66&sid=7D1E6EEC2019967D&eid=F18BA6286A889C1C&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=0