Preliminary Results on PHB Production by Recombinant Cyanobacterium in Line with Green Chemical Industry
绿色化工新例—利用基因重组蓝细菌生产PHB的初步结果

Based on the understanding that the cyanobacterial glycogen biosynthesis is a competitive pathway to cyanobacterial PHB biosynthesis, a genetically engineered cyanobacterium was constructed by partially replacing the agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis by an erythromycin resistance cassette. The obtained mutant propagated faster and the final biomass was higher against its wild-type species, which indicated a much higher photosynthetic efficiency in the mutant. No glycogen was detected in the mutant. Under the photoautotrophic condition, the intracellular PHB content was improved to 15% of the dry weight as compared to 3.4% in the wild-type strain.