%0 Journal Article
%T Preliminary Results on PHB Production by Recombinant Cyanobacterium in Line with Green Chemical Industry<br>绿色化工新例—利用基因重组蓝细菌生产PHB的初步结果
%A WU Gui-fang
%A SHEN Zhong-yao
%A <br>吴桂芳
%A 沈忠耀
%J 过程工程学报
%D 2002
%I 
%X Based on the understanding that the cyanobacterial glycogen biosynthesis is a competitive pathway to cyanobacterial PHB biosynthesis, a genetically engineered cyanobacterium was constructed by partially replacing the agp gene encoding the ADP-glucose pyrophosphorylase involved in cyanobacterial glycogen synthesis by an erythromycin resistance cassette. The obtained mutant propagated faster and the final biomass was higher against its wild-type species, which indicated a much higher photosynthetic efficiency in the mutant. No glycogen was detected in the mutant. Under the photoautotrophic condition, the intracellular PHB content was improved to 15% of the dry weight as compared to 3.4% in the wild-type strain.
%K green chemical industry
%K cyanobacteria
%K PHB
%K recombination<br>绿色化工
%K 蓝细菌
%K PHB
%K 基因重组
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=0516D9362609094D&yid=C3ACC247184A22C1&vid=0B39A22176CE99FB&iid=38B194292C032A66&sid=9F8C5EF901EA1E7E&eid=CAA7BAE04CB631A1&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=13