Synchronous Growth of Vero Cells with Double Thymidine Block
用胸腺嘧啶核苷双阻断法实现Vero细胞的同步化生长

The optimum conditions of synchronous growth of Vero cells with double thymidine (TdR) block were investigated, including the concentration of TdR, treatment time and recovery time. The proper operation procedure is as follows: exponentially growing Vero cells were arrested with 2 mmol/L TdR for 11 h, cultured in normal medium for 14 h and arrested with TdR for another 11 h. In this way, the Synchronous Index (SI) reached 65.0% for the Vero cells culture in bottles and 60.0% for Vero cells growing on Cytodex-3 microcarriers in spinner flasks.