%0 Journal Article
%T Synchronous Growth of Vero Cells with Double Thymidine Block<br>用胸腺嘧啶核苷双阻断法实现Vero细胞的同步化生长
%A GAO Hong-liang
%A CONG Wei
%A ZHI Wen-bo
%A OUYANG Fan
%A SHAO Man-jun
%A <br>高红亮
%A 丛威
%A 郅文波
%A 欧阳藩
%A 邵曼君
%J 过程工程学报
%D 2001
%I 
%X The optimum conditions of synchronous growth of Vero cells with double thymidine (TdR) block were investigated, including the concentration of TdR, treatment time and recovery time. The proper operation procedure is as follows: exponentially growing Vero cells were arrested with 2 mmol/L TdR for 11 h, cultured in normal medium for 14 h and arrested with TdR for another 11 h. In this way, the Synchronous Index (SI) reached 65.0% for the Vero cells culture in bottles and 60.0% for Vero cells growing on Cytodex-3 microcarriers in spinner flasks.
%K Vero cell
%K synchronous growth
%K double thymidine block<br>Vero细胞
%K 同步化
%K 胸腺嘧啶核苷双阻断法
%K 细胞生长
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=DAA7B5A7F51F4C40&yid=14E7EF987E4155E6&vid=CA4FD0336C81A37A&iid=CA4FD0336C81A37A&sid=5C3443B19473A746&eid=09ABD5535D9B6D45&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=10