Screening and Culture of Alkaline Lipase-produced Strain Pseudomonas sp. ZJU-02
产碱性脂肪酶菌株的筛选和培养

A strain Pseudomonas sp. ZJU-02 was screened from 7 strains. The strain had high lipase producing ability proved by the Rodamine B plate method. The results in shake flask cultivation showed that the suitable lipase producing media were: corn steep 3%, vegetable oil 1%, K2HPO4 0.1%, KCl 0.05%, MgSO4 0.05%, Tween 80 0.05%. Under optimal culture conditions (26oC and pH 6.5), the maximal lipase activity could reach 86 IU/ml. This enzyme was stable under 70oC and pH 7~10.5. The optimal conditions for the enzymatic reaction were 40oC and pH 9.5. This enzyme showed a good potential application in detergent and tan industry.