%0 Journal Article
%T Screening and Culture of Alkaline Lipase-produced Strain Pseudomonas sp. ZJU-02<br>产碱性脂肪酶菌株的筛选和培养
%A HONG Jun
%A XIA Li-ming
%A <br>洪骏
%A 夏黎明
%J 过程工程学报
%D 2003
%I 
%X A strain Pseudomonas sp. ZJU-02 was screened from 7 strains. The strain had high lipase producing ability proved by the Rodamine B plate method. The results in shake flask cultivation showed that the suitable lipase producing media were: corn steep 3%, vegetable oil 1%, K2HPO4 0.1%, KCl 0.05%, MgSO4 0.05%, Tween 80 0.05%. Under optimal culture conditions (26oC and pH 6.5), the maximal lipase activity could reach 86 IU/ml. This enzyme was stable under 70oC and pH 7~10.5. The optimal conditions for the enzymatic reaction were 40oC and pH 9.5. This enzyme showed a good potential application in detergent and tan industry.
%K alkali lipase production
%K strain screen
%K enzymatic characteristics<br>碱性脂肪酶
%K 菌种筛选
%K 酶学特性
%K RodamineB平板筛选法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=88F03CF8888E0417&yid=D43C4A19B2EE3C0A&vid=38B194292C032A66&iid=94C357A881DFC066&sid=B7ACE2F11789CAE0&eid=4BEA9A781F286FC6&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=9&reference_num=10