The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Box-Behnken design. The important nutrient sources in the culture medium, identified by the initial screening method of Placket-Burman, were wheat bran, yeast extract, NaNO 3, MgSO 4, and CaCl 2. The optimal amounts (in g/L) for maximum production of xylanase were: wheat bran, 32.8; yeast extract, 1.02; NaNO 3, 12.71; MgSO 4, 0.96; and CaCl 2, 1.04. Using this statistical experimental design, the xylanase production under optimal condition reached 46.50 U/mL and an increase in xylanase activity of 1.34-fold was obtained compared with the original medium for fermentation carried out in a 30-L bioreactor.
References
[1]
Diaz, A.B.; Bolivar, J.; de Ory, I.; Caro, I.; Blandino, A. Applicability of enzymatic extracts obtained by solid state fermentation on grape pomace and orange peels mixtures in must clarification. Lwt-Food Sci. Technol 2011, 44, 840–846.
[2]
Hang, Y.D.; Woodams, E.E. Xylanolytic activity of commercial juice-processing enzyme preparations. Lett. Appl. Microbiol 1997, 24, 389–392.
[3]
Bhushan, S.; Kalia, K.; Sharma, M.; Singh, B.; Ahuja, P.S. Processing of apple pomace for bioactive molecules. Crit. Rev. Biotechnol 2008, 28, 285–296.
[4]
Das, M.; Gupta, S.; Kapoor, V.; Banerjee, R.; Bal, S. Enzymatic polishing of rice—A new processing technology. Lwt-Food Sci. Technol 2008, 41, 2079–2084.
[5]
Biely, P. Microbial xylanolytic systems. Trends Biotechnol 1985, 3, 286–290.
[6]
Viikari, L.; Kantelinen, A.; Sundquist, J.; Linko, M. Xylanases in bleaching: From an idea to the industry. FEMS Microbiol. Rev 1994, 13, 335–350.
[7]
Daneault, C.; Leduc, C.; Valade, J.L. The use of xylanases in kraft pulp bleaching—A review. Tappi J 1994, 77, 125–131.
Wang, H.Y. Effects of rhamnolipid on the cellulase and xylanase in hydrolysis of wheat straw. Bioresour. Technol 2011, 102, 6515–6521.
[11]
Sapag, A.; Wouters, J.; Lambert, C.; de Ioannes, P.; Eyzaguirre, J.; Depiereux, E. The endoxylanases from family 11: Computer analysis of protein sequences reveals important structural and phylogenetic relationships. J. Biotechnol 2002, 95, 109–131.
[12]
Lemos, J.L.S.; Fontes, M.C.D.; Pereira, N. Xylanase production by Aspergillus awamori in solid-state fermentation and influence of different nitrogen sources. Appl. Biochem. Biotechnol 2001, 91, 681–689.
[13]
Gupta, S.; Bhushan, B.; Hoondal, G.S. Enhanced production of xylanase from Staphylococcus sp SG-13 using amino acids. World J. Microb. Biotechnol 1999, 15, 511–512.
[14]
Beg, Q.K.; Bhushan, B.; Kapoor, M.; Hoondal, G.S. Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11-3. J. Ind. Microbiol. Biotechnol 2000, 24, 396–402.
[15]
Plackett, R.L.; Burman, J.P. The design of optimum multifactorial experiments. Biometrika 1946, 37, 305–325.
[16]
Salihu, A.; Alam, M.Z.; AbdulKarim, M.I.; Salleh, H.M. Optimization of lipase production by Candida cylindracea in palm oil mill effluent based medium using statistical experimental design. J. Mol. Catal. Enzym 2011, 69, 66–73.
[17]
Cui, F.J.; Liu, Z.Q.; Li, Y.; Ping, L.F.; Ping, L.Y.; Zhang, Z.C.; Lin, L.; Dong, Y.; Huang, D.M. Production of mycelial biomass and exo-polymer by Hericium erinaceus CZ-2: Optimization of nutrients levels using response surface methodology. Biotechnol. Bioproc 2010, 15, 299–307.
[18]
Periyasamy, A.K.; Muthu, M.; Kannan, V. Optimization of nutrients for the production of RNase by Bacillus firmus VKPACU1 using response surface methodology. Biotechnol. Bioprocess 2010, 14, 202–206.
[19]
Chen, X.S.; Tang, L.; Li, S.; Liao, L.J.; Zhang, J.H.; Mao, Z.G. Optimization of medium for enhancement of epsilon-Poly-l-Lysine production by Streptomyces sp. M-Z18 with glycerol as carbon source. Bioresour. Technol 2011, 102, 1727–1732.
[20]
Li, Y.; Cui, F.J.; Liu, Z.Q.; Xu, Y.Y.; Zhao, H. Improvement of xylanase production by Penicillium oxalicum ZH-30 using response surface methodology. Enzym. Microb. Technol 2007, 40, 1381–1388.
[21]
Bocchini, D.A.; Alves-Prado, H.F.; Baida, L.C.; Roberto, I.C.; Gomes, E.; da Silva, R. Optimization of xylanase production by Bacillus circulans D1 in submerged fermentation using response surface methodology. Process Biochem 2002, 38, 727–731.
[22]
Li, Y.; Lin, J.; Meng, D.J.; Lu, J.; Gu, G.X.; Mao, Z.G. Effect of pH, cultivation time and substrate concentration on the endoxylanase production by Aspergillus awamori ZH-26 under submerged fermentation using central composite rotary design. Food Technol. Biotechnol 2006, 44, 473–477.
[23]
Liu, W.; Lu, Y.L.; Ma, G.R. Induction and glucose repression of endo-beta-xylanase in the yeast Trichosporon cutaneum SL409. Process Biochem 1999, 34, 67–72.
[24]
Fernandezespinar, M.; Pinaga, F.; Degraaff, L.; Visser, J.; Ramon, D.; Valles, S. Purification, characterization and regulation of the synthesis of an Aspergillus-nidulans acidic xylanase. Appl. Microbiol. Biotechnol 1994, 42, 555–562.
Senthilkumar, S.R.; Ashokkumar, B.; Raj, K.C.; Gunasekaran, P. Optimization of medium composition for alkali-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design. Bioresour. Technol 2005, 96, 1380–1386.
[29]
Ding, C.H.; Jiang, Z.Q.; Li, X.T.; Li, L.T.; Kusakabe, I. High activity xylanase production by Streptomyces olivaceoviridis E-86. World J. Microb. Biotechnol 2004, 20, 7–10.
[30]
Techapun, C.; Sinsuwongwat, S.; Watanabe, M.; Sasaki, K.; Poosaran, N. Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods. Biotechnol. Lett 2002, 24, 1437–1442.
[31]
Box, G.E.; Behnken, D.W. Some new three level designs for the study of quantitative variable. Technometrics 1960, 2, 456–475.
[32]
Cui, F.J.; Li, Y.; Liu, Z.Q.; Zhao, H.; Ping, L.F.; Ping, L.Y. Optimization of fermentation conditions for production of xylanase by a newly isolated strain, Penicillium thiersii ZH-19. World J. Microb. Biotechnol 2009, 25, 721–725.
