%0 Journal Article
%T Optimization of Xylanase Production from Penicillium sp.WX-Z1 by a Two-Step Statistical Strategy: Plackett-Burman and Box-Behnken Experimental Design
%A Fengjie Cui
%A Liming Zhao
%J International Journal of Molecular Sciences
%D 2012
%I MDPI AG
%R 10.3390/ijms130810630
%X The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response surface methodology (RSM) was further optimized for xylanase production by Box-Behnken design. The important nutrient sources in the culture medium, identified by the initial screening method of Placket-Burman, were wheat bran, yeast extract, NaNO 3, MgSO 4, and CaCl 2. The optimal amounts (in g/L) for maximum production of xylanase were: wheat bran, 32.8; yeast extract, 1.02; NaNO 3, 12.71; MgSO 4, 0.96; and CaCl 2, 1.04. Using this statistical experimental design, the xylanase production under optimal condition reached 46.50 U/mL and an increase in xylanase activity of 1.34-fold was obtained compared with the original medium for fermentation carried out in a 30-L bioreactor.
%K Penicillium sp.WX-Z1
%K xylanase
%K nutrient sources
%K Plackett-Burman design
%K Box-Behnken design
%U http://www.mdpi.com/1422-0067/13/8/10630