Screening and Identification of Mouse Biccl RNAi
小鼠Biccl基因RNAi序列的筛选与鉴定

Based on the online bioinformatics analysis and alignment results,three RNAi sequences target to the Mus musculus Bicc1 gene were obtained.The three interference fragments were synthesized and cloned into pRS-Hush shRNA Vector.The Bicc1 eukaryotic expression vector pEGFP-C3-Bicc1 was constructed,tagging the GFP to the N-terminal of the Bicc1 protein.The pEGFP-C3-Bicc1 and three pRS-Hush-RNAi were co-transfected into the cultured HEK-293 cells line,respectively.The two RNAi (pRS-Hush-RNAi-Bicc1-N/-C) that co...