%0 Journal Article
%T Screening and Identification of Mouse Biccl RNAi<br>小鼠Biccl基因RNAi序列的筛选与鉴定
%A ZHOU Liang
%A YANG Jun-Xing
%A <br>周亮
%A 杨君兴
%J 动物学研究
%D 2010
%I Chinese Academy of Sciences
%X Based on the online bioinformatics analysis and alignment results,three RNAi sequences target to the Mus musculus Bicc1 gene were obtained.The three interference fragments were synthesized and cloned into pRS-Hush shRNA Vector.The Bicc1 eukaryotic expression vector pEGFP-C3-Bicc1 was constructed,tagging the GFP to the N-terminal of the Bicc1 protein.The pEGFP-C3-Bicc1 and three pRS-Hush-RNAi were co-transfected into the cultured HEK-293 cells line,respectively.The two RNAi (pRS-Hush-RNAi-Bicc1-N/-C) that co...
%K Mouse Bicc1 gene
%K RNAi
%K pRS-Hush
%K pEGFP-C3-Bicc1
%K Transfect<br>小鼠Bicc1基因
%K RNA干扰
%K pRS-Hush
%K pEGFP-C3-Bicc1
%K 转染
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=A9CE91307C3FDE3F5011FC5CB3290E8A&yid=140ECF96957D60B2&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=656F8C8401D91023&eid=7E8E8B150580E4AB&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=10