Cloning and Functional Analysis of the Full Length cDNA Sequence of Eukaryotic Translation Initiation Factor 5 in Schistosoma japonicum
日本血吸虫真核翻译起始因子5基因的cDNA克隆和免疫保护功能分析(英文)

The expressed sequence tag of eukaryotic translation initiation factor 5 (elF5) from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioin-formatics method. The overlapping sequences were assembled into one that includes the complete open reading frame (GenBank accession number: AY686501). The full-length cDNA of SjelF5 was cloned into a pET-28c( ) vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coli. The recombi-nant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.